This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CPH3567 (pET151_Spt6 1247-1451 R1282H)
catalog :
91816
citations: 1
Reference
Sdano M, Fulcher J, Palani S, Chandrasekharan M, Parnell T, Whitby F, et al. A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription. elife. 2017;6: pubmed publisher
product information
Catalog Number :
91816
Product Name :
CPH3567 (pET151_Spt6 1247-1451 R1282H)
article :
doi10.7554/eLife.28723
id25557
pubmed_id28826505
bacterial resistance :
Ampicillin
cloning :
backbonepET151/D-TOPO
backbone_mutation
backbone_originThermo Fisher Scientific
backbone_size5766
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation
origin :
37
pi :
alt_names
CRE2
SSN20
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7trm
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYGR116W, CRE2, SSN20
geneSPT6
id853011
genbank_ids
NM_001181245.1
mutationchanged arginine 1282 to histidine; deleted residues 1-1246
nameSPT6
shRNA_sequence
size615
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tag6xHis
resistance markers :
2231
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA