This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHIV-H2B-eGFP
catalog :
91776
citations: 3
Reference
Annunziata C, Castoldi F, Jan S, Ang H, Ristovska M, Melini S, et al. A versatile method for the identification of senolytic compounds. Cell Stress. 2023;7:105-111 pubmed publisher
Saydam O, Saydam N. Deficiency of Ku Induces Host Cell Exploitation in Human Cancer Cells. Front Cell Dev Biol. 2021;9:651818 pubmed publisher
Sottile F, Aulicino F, Theka I, Cosma M. Mesenchymal stem cells generate distinct functional hybrids in vitro via cell fusion or entosis. Sci Rep. 2016;6:36863 pubmed publisher
product information
Catalog Number :
91776
Product Name :
pHIV-H2B-eGFP
article :
doi10.1038/srep36863
id24405
pubmed_id27827439
bacterial resistance :
Ampicillin
cloning :
backboneHIV-H2B-mRFP
backbone_mutation
backbone_origin
backbone_size8054
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The HIV-H2BeGFP plasmid was cloned by replacing mRFP in HIV-H2B-mRFP (Bryan Welm & Zena Werb Addgene plasmid # 18982 ) with H2BeGFP from H2B-GFP plasmid (Geoff Wahl Addgene plasmid # 11680) to generate a bicistronic lentiviral vector.
growth strain :
Expresses H2B protein tagged with eGFP
origin :
37
pi :
alt_names
HIST1H2BJ
cloning
clone_methodRestriction Enzyme
cloning_site_3ClaI
cloning_site_5EcoRI
promoterEF1a
sequencing_primer_3atcgatGTCGCGGCCGCTTTACTTGTACA
sequencing_primer_5atcatgaattcgtttgtgaacgacattttcgagcg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesH2B/r, H2BFR, H2BJ, HIST1H2BJ
geneH2BC11
id8970
genbank_ids
mutation
nameHuman Histone H2B / eGFP
shRNA_sequence
size1135
species
9606
Homo sapiens
tags
locationC terminal on insert
tageGFP
plasmid copy :
We used the H2B-GFP plasmid from Geoff Wahl (Addgene plasmid # 11680) as donor vector
resistance markers :
3459
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA