This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFUSEss-chimeric-IgM-mouse/human_M18
catalog :
91738
citations: 1
Reference
Klaus T, Stalinska K, Czaplicki D, Mak P, Skupien Rabian B, Kedracka Krok S, et al. Mouse Antibody of IgM Class is Prone to Non-Enzymatic Cleavage between CH1 and CH2 Domains. Sci Rep. 2018;8:519 pubmed publisher
product information
Catalog Number :
91738
Product Name :
pFUSEss-chimeric-IgM-mouse/human_M18
article :
doi10.1038/s41598-017-19003-4
id25541
pubmed_id29323348
bacterial resistance :
Bleocin (Zeocin)
cloning :
backbonepFUSEss-CHIg-mM
backbone_mutationResidues 203-239 (EU numbering) were exchanged to human homologue sequence. The residues correspond to the linker between CH1 and CH2.
backbone_originInvivogen
backbone_size4852
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence.
growth temp :
Top10 or DH5alpha could be selected on LB LOW SALT with 35-50 micrograms/ml Zeocin.
origin :
37
pi :
alt_names
IgM, IGHM, mouse IgM (immunoglobulin M) heavy chain
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5EcoRI
promoterhEF1-HTLV prom
sequencing_primer_3EBV-rev
sequencing_primer_5RVprimer3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesIgh-6, Igh-M, Igh6, Igm, muH
geneIghm
id16019
genbank_ids
mutationResidues 203-239 (EU numbering) were exchanged to human homologue sequence.
nameimmunoglobulin heavy constant mu
shRNA_sequence
size367
species
10090
Mus musculus
tags
resistance markers :
2286
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA