This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE puro K-Ras V12
catalog :
9052
citations: 22
Reference
Lee D, Kim I, Lee H, Seo M, Cho M, Lee H, et al. Akt enhances the vulnerability of cancer cells to VCP/p97 inhibition-mediated paraptosis. Cell Death Dis. 2024;15:48 pubmed publisher
Pal D, Lin Y, Zhan H, Banerjee T, Kuhn J, Providence S, et al. Optogenetic modulation of guanine nucleotide exchange factors of Ras superfamily proteins directly controls cell shape and movement. Front Cell Dev Biol. 2023;11:1195806 pubmed publisher
Ho T, Lee M, Goh H, Ng G, Lee J, Kannan S, et al. Domain-specific p53 mutants activate EGFR by distinct mechanisms exposing tissue-independent therapeutic vulnerabilities. Nat Commun. 2023;14:1726 pubmed publisher
Sturmlechner I, Zhang C, Sine C, van Deursen E, Jeganathan K, Hamada N, et al. p21 produces a bioactive secretome that places stressed cells under immunosurveillance. Science. 2021;374:eabb3420 pubmed publisher
Trayssac M, Clarke C, Stith J, Snider J, Newen N, Gault C, et al. Targeting sphingosine kinase 1 (SK1) enhances oncogene-induced senescence through ceramide synthase 2 (CerS2)-mediated generation of very-long-chain ceramides. Cell Death Dis. 2021;12:27 pubmed publisher
Di Tano M, Raucci F, Vernieri C, Caffa I, Buono R, Fanti M, et al. Synergistic effect of fasting-mimicking diet and vitamin C against KRAS mutated cancers. Nat Commun. 2020;11:2332 pubmed publisher
Morgan C, Hedman A, Li Z, Sacks D. Endogenous IQGAP1 and IQGAP3 do not functionally interact with Ras. Sci Rep. 2019;9:11057 pubmed publisher
Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, et al. PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth. Cancer Cell. 2018;33:355-367.e7 pubmed publisher
Lee J, Snyder E, Liu Y, Gu X, Wang J, Flowers B, et al. Reconstituting development of pancreatic intraepithelial neoplasia from primary human pancreas duct cells. Nat Commun. 2017;8:14686 pubmed publisher
Welsch M, Kaplan A, Chambers J, Stokes M, Bos P, Zask A, et al. Multivalent Small-Molecule Pan-RAS Inhibitors. Cell. 2017;168:878-889.e29 pubmed publisher
Chen N, Neesse A, Dyck M, Steuber B, Koenig A, Lubeseder Martellato C, et al. Context-Dependent Epigenetic Regulation of Nuclear Factor of Activated T Cells 1 in Pancreatic Plasticity. Gastroenterology. 2017;152:1507-1520.e15 pubmed publisher
Seo J, Choi J, Lee S, Sung S, Yoo H, Kang M, et al. Autophagy is required for PDAC glutamine metabolism. Sci Rep. 2016;6:37594 pubmed publisher
Sung S, Choi J, Cheong H. Catabolic pathways regulated by mTORC1 are pivotal for survival and growth of cancer cells expressing mutant Ras. Oncotarget. 2015;6:40405-17 pubmed publisher
Vasseur R, Skrypek N, Duchêne B, Renaud F, Martínez Maqueda D, Vincent A, et al. The mucin MUC4 is a transcriptional and post-transcriptional target of K-ras oncogene in pancreatic cancer. Implication of MAPK/AP-1, NF-κB and RalB signaling pathways. Biochim Biophys Acta. 2015;1849:1375-84 pubmed publisher
Zou Z, Nie P, Li Y, Hou B, Rui Li -, Shi X, et al. Synergistic induction of apoptosis by salinomycin and gefitinib through lysosomal and mitochondrial dependent pathway overcomes gefitinib resistance in colorectal cancer. Oncotarget. 2017;8:22414-22432 pubmed publisher
Ricoult S, Yecies J, Ben Sahra I, Manning B. Oncogenic PI3K and K-Ras stimulate de novo lipid synthesis through mTORC1 and SREBP. Oncogene. 2016;35:1250-60 pubmed publisher
Hamilton G, Abraham A, Morton J, Sampson O, Pefani D, Khoronenkova S, et al. AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget. 2014;5:6142-67 pubmed
Patel A, Burton D, Halvorsen K, Balkan W, Reiner T, Perez Stable C, et al. MutT Homolog 1 (MTH1) maintains multiple KRAS-driven pro-malignant pathways. Oncogene. 2015;34:2586-96 pubmed publisher
Chen J, Bi H, Hou J, Zhang X, Zhang C, Yue L, et al. Atorvastatin overcomes gefitinib resistance in KRAS mutant human non-small cell lung carcinoma cells. Cell Death Dis. 2013;4:e814 pubmed publisher
Magudia K, Lahoz A, Hall A. K-Ras and B-Raf oncogenes inhibit colon epithelial polarity establishment through up-regulation of c-myc. J Cell Biol. 2012;198:185-94 pubmed publisher
Whitsett T, CHENG E, Inge L, Asrani K, Jameson N, Hostetter G, et al. Elevated expression of Fn14 in non-small cell lung cancer correlates with activated EGFR and promotes tumor cell migration and invasion. Am J Pathol. 2012;181:111-20 pubmed publisher
Chauhan S, Boyd D. Regulation of u-PAR gene expression by H2A.Z is modulated by the MEK-ERK/AP-1 pathway. Nucleic Acids Res. 2012;40:600-13 pubmed publisher
product information
Catalog Number :
9052
Product Name :
pBABE puro K-Ras V12
article :
doi
id355
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepBabe puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Retroviral expression of a constitutively active form of K-Ras
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3pBABE-3
sequencing_primer_5pBABE-5
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutationG12V. Constitutively active.
nameKRAS
shRNA_sequence
size570
species
9606
Homo sapiens
tags
resistance markers :
184
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA