1068 pGL3 FasL promoter (no SV40 prom) | Addgene 9029 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

1068 pGL3 FasL promoter (no SV40 prom)

catalog :

9029

citations: 1

Reference
Nakamura N, Ramaswamy S, Vazquez F, Signoretti S, Loda M, Sellers W. Forkhead transcription factors are critical effectors of cell death and cell cycle arrest downstream of PTEN. Mol Cell Biol. 2000;20:8969-82 pubmed

product information

Catalog Number :

9029

Product Name :

1068 pGL3 FasL promoter (no SV40 prom)

article :

doi	10.1128/MCB.20.23.8969-8982.2000
id	353
pubmed_id	11073996

bacterial resistance :

Ampicillin

cloning :

backbone

pGL3 promoter

backbone_mutation

backbone_origin

backbone_size

4810

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

and 5'-GGGG AGATCTGCTTTGTATTTCACAATGTTTTCATTTTCATTGTTTGCCCAG TTTATTTATTT-3', containing the forkhead site of the FasL promoter, were phosphorylated, annealed, and ligated to pGL3-promoter restricted with BglII and NheI to give pGL3-promoter-FasL. This plasmid was subsequently restricted with BglII and HindIII, blunted, and ligated to remove the simian virus 40
5'-GCGCGCTAGCGTGACAGAGTGAGACTCTGTCTCTAT
TTAAATAAATAAGTAAATAAATAAAC-3'
Oligonucleotides

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	NheI
promoter
sequencing_primer_3
sequencing_primer_5	RVprimer3
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, TNFSF6, TNLG1A
gene	FASLG
id	356

genbank_ids

mutation

Removed the SV40 promoter from pGL3

name

Fas ligand promoter

shRNA_sequence

size

100

species

9606

Homo sapiens