This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ALARM NMR
catalog :
90209
citations: 2
Reference
Dahlin J, Cuellar M, Singh G, Nelson K, Strasser J, Rappe T, et al. ALARM NMR for HTS triage and chemical probe validation. Curr Protoc Chem Biol. 2018;10:91-117 pubmed publisher
Dahlin J, Nelson K, Strasser J, Barsyte Lovejoy D, Szewczyk M, Organ S, et al. Assay interference and off-target liabilities of reported histone acetyltransferase inhibitors. Nat Commun. 2017;8:1527 pubmed publisher
product information
Catalog Number :
90209
Product Name :
ALARM NMR
article :
doi10.1038/s41467-017-01657-3
id25427
pubmed_id29142305
bacterial resistance :
Kanamycin
cloning :
backbonepET28b+
backbone_mutation
backbone_originNovagen
backbone_size5368
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Transform into Rosetta DE3 for protein expression
growth strain :
Expresses the human La antigen for use in ALARM NMR
origin :
37
pi :
alt_names
SSB, La, L/Sjogren syndrome antigen B
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoterT7 promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLARP3, La, La/SSB
geneSSB
id6741
genbank_ids
NM_003142.4
mutationcontains amino acids 100-324
nameLa antigen
shRNA_sequence
size813
species
9606
Homo sapiens
tags
locationN terminal on insert
tag6xHis
locationC terminal on insert
tag6xHis
resistance markers :
3376
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA