This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
1271 pBabe puroL Myr HA Akt2
catalog :
9018
citations: 2
Reference
Quambusch L, Depta L, Landel I, Lubeck M, Kirschner T, Nabert J, et al. Cellular model system to dissect the isoform-selectivity of Akt inhibitors. Nat Commun. 2021;12:5297 pubmed publisher
Hamilton G, Abraham A, Morton J, Sampson O, Pefani D, Khoronenkova S, et al. AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget. 2014;5:6142-67 pubmed
product information
Catalog Number :
9018
Product Name :
1271 pBabe puroL Myr HA Akt2
article :
doi
id349
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepBabe puro L
backbone_mutation
backbone_origin
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The sequence of the HA tag in this plasmid is YAYDVPDYA instead of YPYDVPDYA. Not all HA antibodies will recognize this sequence. Cell Signaling Technology's HA antibody #2367 will work.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE-3
sequencing_primer_5pBABE-5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA
geneAKT2
id208
genbank_ids
mutation
nameAkt2
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMyr
locationN terminal on backbone
tagHA
resistance markers :
455
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA