This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
1227 pcDNA3 Myr HA Akt2
catalog :
9016
citations: 17
Reference
Choi Y, Lee K, Yoon J, Cho S, Kim Y, Jeong M, et al. SH003 and Docetaxel Show Synergistic Anticancer Effects by Inhibiting EGFR Activation in Triple-Negative Breast Cancer. Biomed Res Int. 2022;2022:3647900 pubmed publisher
Lu W, Omari R, Ray H, Wang J, Williams I, Jacobs C, et al. AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation. FEBS J. 2022;289:3876-3893 pubmed publisher
Lyu C, Ye Y, Lensing M, Wagner K, Weigel R, Chen S. Targeting Gi/o protein-coupled receptor signaling blocks HER2-induced breast cancer development and enhances HER2-targeted therapy. JCI Insight. 2021;6: pubmed publisher
Larson Casey J, Gu L, Davis D, Cai G, Ding Q, He C, et al. Post-translational regulation of PGC-1α modulates fibrotic repair. FASEB J. 2021;35:e21675 pubmed publisher
Shen Q, Zhu H, Lei Q, Chen L, Yang D, Sui W. MicroRNA‑149‑3p inhibits cell proliferation by targeting AKT2 in oral squamous cell carcinoma. Mol Med Rep. 2021;23: pubmed publisher
Nam H, Jeon S, An H, Yoo J, Lee H, Lee S, et al. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification. elife. 2019;8: pubmed publisher
Rietscher K, Keil R, Jordan A, Hatzfeld M. 14-3-3 proteins regulate desmosomal adhesion via plakophilins. J Cell Sci. 2018;131: pubmed publisher
Figlia G, Norrmen C, Pereira J, Gerber D, Suter U. Dual function of the PI3K-Akt-mTORC1 axis in myelination of the peripheral nervous system. elife. 2017;6: pubmed publisher
Hedl M, Yan J, Abraham C. IRF5 and IRF5 Disease-Risk Variants Increase Glycolysis and Human M1 Macrophage Polarization by Regulating Proximal Signaling and Akt2 Activation. Cell Rep. 2016;16:2442-55 pubmed publisher
Mehta A, Comunale M, Rawat S, Casciano J, Lamontagne J, Herrera H, et al. Intrinsic hepatocyte dedifferentiation is accompanied by upregulation of mesenchymal markers, protein sialylation and core alpha 1,6 linked fucosylation. Sci Rep. 2016;6:27965 pubmed publisher
Miao L, Yang L, Huang H, Liang F, Ling C, Hu Y. mTORC1 is necessary but mTORC2 and GSK3? are inhibitory for AKT3-induced axon regeneration in the central nervous system. elife. 2016;5:e14908 pubmed publisher
Shi Q, Hoffman B, Liu Q. PI3K-Akt signaling pathway upregulates hepatitis C virus RNA translation through the activation of SREBPs. Virology. 2016;490:99-108 pubmed publisher
Lee S, Craig B, Romain C, Qiao J, Chung D. Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation. Cancer Lett. 2014;355:210-6 pubmed publisher
Sahlberg S, Spiegelberg D, Glimelius B, Stenerlow B, Nestor M. Evaluation of cancer stem cell markers CD133, CD44, CD24: association with AKT isoforms and radiation resistance in colon cancer cells. PLoS ONE. 2014;9:e94621 pubmed publisher
Elloul S, Kedrin D, Knoblauch N, Beck A, Toker A. The adherens junction protein afadin is an AKT substrate that regulates breast cancer cell migration. Mol Cancer Res. 2014;12:464-76 pubmed publisher
Qiao J, Lee S, Paul P, Qiao L, Taylor C, Schlegel C, et al. Akt2 regulates metastatic potential in neuroblastoma. PLoS ONE. 2013;8:e56382 pubmed publisher
Wang H, Fan L, Wei J, Weng Y, Zhou L, Shi Y, et al. Akt mediates metastasis-associated gene 1 (MTA1) regulating the expression of E-cadherin and promoting the invasiveness of prostate cancer cells. PLoS ONE. 2012;7:e46888 pubmed publisher
product information
Catalog Number :
9016
Product Name :
1227 pcDNA3 Myr HA Akt2
article :
doi
id349
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The sequence of the HA tag in this plasmid is YAYDVPDYA instead of YPYDVPDYA. Not all HA antibodies will recognize this sequence. Cell Signaling Technology's HA antibody #2367 will work.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA
geneAKT2
id208
genbank_ids
mutation
nameAkt2
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMyr
locationN terminal on backbone
tagHA
resistance markers :
455
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA