This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
1072 pBabe puroL myr Akt K179M T308A S473A
catalog :
9015
citations: 1
Reference
DiRenzo J, Signoretti S, Nakamura N, Rivera Gonzalez R, Sellers W, Loda M, et al. Growth factor requirements and basal phenotype of an immortalized mammary epithelial cell line. Cancer Res. 2002;62:89-98 pubmed
product information
Catalog Number :
9015
Product Name :
1072 pBabe puroL myr Akt K179M T308A S473A
article :
doi
id352
pubmed_id11782364
bacterial resistance :
Ampicillin
cloning :
backbonepBabe puro L
backbone_mutation
backbone_origin
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The sequence of the HA tag in this plasmid is YAYDVPDYA instead of YPYDVPDYA. Not all HA antibodies will recognize this sequence. Cell Signaling Technology's HA antibody #2367 will work.
origin :
37
pi :
alt_names
Akt1
Akt
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5pBabe5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
mutationK179M T308A S473A
nameAkt1 K179M T308A S473A
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMyr
locationN terminal on backbone
tagHA
resistance markers :
455
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA