This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMMB67EH-Spy
catalog :
90102
citations: 3
Reference
Ide H, Hayashida Y, Morimoto Y. Visualization of c-di-GMP in multicellular Dictyostelium stages. Front Cell Dev Biol. 2023;11:1237778 pubmed publisher
Hariharan V, Yadav R, Thakur C, Singh A, Gopinathan R, Singh D, et al. Cyclic di-GMP sensing histidine kinase PdtaS controls mycobacterial adaptation to carbon sources. FASEB J. 2021;35:e21475 pubmed publisher
Christen M, Kulasekara H, Christen B, Kulasekara B, Hoffman L, Miller S. Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. Science. 2010;328:1295-7 pubmed publisher
product information
Catalog Number :
90102
Product Name :
pMMB67EH-Spy
article :
doi10.1126/science.1188658
id7260
pubmed_id20522779
bacterial resistance :
Gentamicin
cloning :
backbonepMMB67EH
backbone_mutation
backbone_origin
backbone_size9449
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Other
Broad-host range
growth strain :
IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.
origin :
37
pi :
alt_names
Spy
cyclic-di-GMP biosensor
YFP-YcgR-CFP
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5EcoRI
promoterPtac
sequencing_primer_3pBAD-Rvs
sequencing_primer_5none
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb1194, ECK1182, JW1183
geneycgR
id947609
genbank_ids
1253317
mutation
nameYcgR
shRNA_sequence
size2187
species
tags
resistance markers :
3372
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA