This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
1036 pcDNA3 Myr HA Akt1
catalog :
9008
citations: 50
Reference
Lee W, Liao S, Huang Y, Hou M, Lan K. Akt1 is involved in HCV release by promoting endoplasmic reticulum-to-endosome transition of infectious virions. Life Sci. 2024;338:122412 pubmed publisher
Elakad O, H xe4 upl B, Labitzky V, Yao S, K xfc ffer S, von Hammerstein Equord A, et al. Activation of CD44/PAK1/AKT signaling promotes resistance to FGFR1 inhibition in squamous-cell lung cancer. NPJ Precis Oncol. 2022;6:52 pubmed publisher
Tian W, Yuan H, Qin S, Liu W, Zhang B, Gu L, et al. Kaiso phosphorylation at threonine 606 leads to its accumulation in the cytoplasm, reducing its transcriptional repression of the tumour suppressor CDH1. Mol Oncol. 2022;16:3192-3209 pubmed publisher
Shrestha Bhattarai T, Shamu T, Gorelick A, Chang M, Chakravarty D, Gavrila E, et al. AKT mutant allele-specific activation dictates pharmacologic sensitivities. Nat Commun. 2022;13:2111 pubmed publisher
Lu W, Omari R, Ray H, Wang J, Williams I, Jacobs C, et al. AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation. FEBS J. 2022;289:3876-3893 pubmed publisher
Cha Y, Kim T, Jeon J, Jang Y, Kim P, Lopes C, et al. SIRT2 regulates mitochondrial dynamics and reprogramming via MEK1-ERK-DRP1 and AKT1-DRP1 axes. Cell Rep. 2021;37:110155 pubmed publisher
Lupse B, Annamalai K, Ibrahim H, Kaur S, Geravandi S, Sarma B, et al. Inhibition of PHLPP1/2 phosphatases rescues pancreatic β-cells in diabetes. Cell Rep. 2021;36:109490 pubmed publisher
Nalairndran G, Chung I, Abdul Razack A, Chung F, Hii L, Lim W, et al. Inhibition of Janus Kinase 1 synergizes docetaxel sensitivity in prostate cancer cells. J Cell Mol Med. 2021;: pubmed publisher
Lee B, Boyer J, Burnett G, Thottumkara A, Tibrewal N, Wilson S, et al. Selective inhibitors of mTORC1 activate 4EBP1 and suppress tumor growth. Nat Chem Biol. 2021;: pubmed publisher
Wei J, Leung K, Truillet C, Ruggero D, Wells J, Evans M. Profiling the surfaceome identifies therapeutic targets for cells with hyperactive mTORC1 signaling. Mol Cell Proteomics. 2019;: pubmed publisher
Chen G, Park D, Magis A, Behera M, Ramalingam S, Owonikoko T, et al. Mcl-1 Interacts with Akt to Promote Lung Cancer Progression. Cancer Res. 2019;79:6126-6138 pubmed publisher
Wallroth A, Koch P, Marat A, Krause E, Haucke V. Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1. Nat Cell Biol. 2019;21:1093-1101 pubmed publisher
Wu X, Li S, Hu X, Xiang X, Halloran M, Yang L, et al. mTOR Signaling Upregulates CDC6 via Suppressing miR-3178 and Promotes the Loading of DNA Replication Helicase. Sci Rep. 2019;9:9805 pubmed publisher
Kuang J, Guo Y, Chen Y, Wang J, Duan J, He X, et al. Connexin 43 C-terminus directly inhibits the hyperphosphorylation of Akt/ERK through protein-protein interactions in glioblastoma. Cancer Sci. 2018;109:2611-2622 pubmed publisher
LI Y, Du L, Aldana Masangkay G, Wang X, Urak R, Forman S, et al. Regulation of miR-34b/c-targeted gene expression program by SUMOylation. Nucleic Acids Res. 2018;: pubmed publisher
Chia K, Mazzolini J, Mione M, Sieger D. Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain. elife. 2018;7: pubmed publisher
Osswald C, Xie L, Guan H, Herrmann F, Pick S, Vogel M, et al. Fine-tuning of FOXO3A in cHL as a survival mechanism and a hallmark of abortive plasma cell differentiation. Blood. 2018;131:1556-1567 pubmed publisher
Wang L, Guo W, Ma J, Dai W, Liu L, Guo S, et al. Aberrant SIRT6 expression contributes to melanoma growth: Role of the autophagy paradox and IGF-AKT signaling. Autophagy. 2018;14:518-533 pubmed publisher
Gallagher S, Gunatilake D, Beaumont K, Sharp D, Tiffen J, Heinemann A, et al. HDAC inhibitors restore BRAF-inhibitor sensitivity by altering PI3K and survival signalling in a subset of melanoma. Int J Cancer. 2018;142:1926-1937 pubmed publisher
Figlia G, Norrmen C, Pereira J, Gerber D, Suter U. Dual function of the PI3K-Akt-mTORC1 axis in myelination of the peripheral nervous system. elife. 2017;6: pubmed publisher
Zhou X, Liu W, Hu X, Dorrance A, Garzon R, Houghton P, et al. Regulation of CHK1 by mTOR contributes to the evasion of DNA damage barrier of cancer cells. Sci Rep. 2017;7:1535 pubmed publisher
Lee H, Lo K, Wei W, Tsao S, Chung G, Ibrahim M, et al. Oncogenic S1P signalling in EBV-associated nasopharyngeal carcinoma activates AKT and promotes cell migration through S1P receptor 3. J Pathol. 2017;242:62-72 pubmed publisher
Soo H, Chung F, Lim K, Yap V, Bradshaw T, Hii L, et al. Cudraflavone C Induces Tumor-Specific Apoptosis in Colorectal Cancer Cells through Inhibition of the Phosphoinositide 3-Kinase (PI3K)-AKT Pathway. PLoS ONE. 2017;12:e0170551 pubmed publisher
Mayrhofer M, Gourain V, Reischl M, Affaticati P, Jenett A, Joly J, et al. A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK- and PI3K-induced malignant growth. Dis Model Mech. 2017;10:15-28 pubmed publisher
Choi H, Shin J, Leem D, Kim S, Cho W, Lee K. 6-(3,4-Dihydro-1H-isoquinoline-2-yl)-N-(6-methoxypyridine-2-yl) nicotinamide-26 (DIMN-26) decreases cell proliferation by induction of apoptosis and downregulation of androgen receptor signaling in human prostate cancer cells. Chem Biol Interact. 2016;260:196-207 pubmed publisher
Swaminathan G, Zhu W, Plowey E. BECN1/Beclin 1 sorts cell-surface APP/amyloid ? precursor protein for lysosomal degradation. Autophagy. 2016;12:2404-2419 pubmed
Diez H, Benitez M, Fernandez S, Torres Aleman I, Garrido J, Wandosell F. Class I PI3-kinase or Akt inhibition do not impair axonal polarization, but slow down axonal elongation. Biochim Biophys Acta. 2016;1863:2574-2583 pubmed publisher
Mehta A, Comunale M, Rawat S, Casciano J, Lamontagne J, Herrera H, et al. Intrinsic hepatocyte dedifferentiation is accompanied by upregulation of mesenchymal markers, protein sialylation and core alpha 1,6 linked fucosylation. Sci Rep. 2016;6:27965 pubmed publisher
Tiong K, Tan B, Choo H, Chung F, Hii L, Tan S, et al. Fibroblast growth factor receptor 4 (FGFR4) and fibroblast growth factor 19 (FGF19) autocrine enhance breast cancer cells survival. Oncotarget. 2016;7:57633-57650 pubmed publisher
Lv D, Liu J, Guo L, Wu D, Matsumoto K, Huang L. PRAS40 deregulates apoptosis in Ewing sarcoma family tumors by enhancing the insulin receptor/Akt and mTOR signaling pathways. Am J Cancer Res. 2016;6:486-97 pubmed
Panda P, Behera B, Meher B, Das D, Mukhopadhyay S, Sinha N, et al. Abrus Agglutinin, a type II ribosome inactivating protein inhibits Akt/PH domain to induce endoplasmic reticulum stress mediated autophagy-dependent cell death. Mol Carcinog. 2017;56:389-401 pubmed publisher
Miao L, Yang L, Huang H, Liang F, Ling C, Hu Y. mTORC1 is necessary but mTORC2 and GSK3? are inhibitory for AKT3-induced axon regeneration in the central nervous system. elife. 2016;5:e14908 pubmed publisher
Shi Q, Hoffman B, Liu Q. PI3K-Akt signaling pathway upregulates hepatitis C virus RNA translation through the activation of SREBPs. Virology. 2016;490:99-108 pubmed publisher
Lu Q, Yan S, Sun H, Wang W, Li Y, Yang X, et al. Akt inhibition attenuates rasfonin-induced autophagy and apoptosis through the glycolytic pathway in renal cancer cells. Cell Death Dis. 2015;6:e2005 pubmed publisher
Singh B, Sinha R, Zhou J, Tripathi M, Ohba K, Wang M, et al. Hepatic FOXO1 Target Genes Are Co-regulated by Thyroid Hormone via RICTOR Protein Deacetylation and MTORC2-AKT Protein Inhibition. J Biol Chem. 2016;291:198-214 pubmed publisher
Zhang H, Alshareef A, Wu C, Li S, Jiao J, Cao H, et al. Loss of miR-200b promotes invasion via activating the Kindlin-2/integrin β1/AKT pathway in esophageal squamous cell carcinoma: An E-cadherin-independent mechanism. Oncotarget. 2015;6:28949-60 pubmed publisher
Xie C, Lu H, Nomura A, Hanse E, Forster C, Parker J, et al. Co-deleting Pten with Rb in retinal progenitor cells in mice results in fully penetrant bilateral retinoblastomas. Mol Cancer. 2015;14:93 pubmed publisher
Kim Y, Seol C, Jung J, Oh S, Hwang K, Kim H, et al. Synergistic Effect of Sulindac and Simvastatin on Apoptosis in Lung Cancer A549 Cells through AKT-Dependent Downregulation of Survivin. Cancer Res Treat. 2015;47:90-100 pubmed publisher
Qi L, Zhu F, Li S, Si L, Hu L, Tian H. Retinoblastoma binding protein 2 (RBP2) promotes HIF-1α-VEGF-induced angiogenesis of non-small cell lung cancer via the Akt pathway. PLoS ONE. 2014;9:e106032 pubmed publisher
Vignarajan S, Xie C, Yao M, Sun Y, Simanainen U, Sved P, et al. Loss of PTEN stabilizes the lipid modifying enzyme cytosolic phospholipase A?? via AKT in prostate cancer cells. Oncotarget. 2014;5:6289-99 pubmed publisher
Li C, Chen J, Lu B, Shi Z, Wang H, Zhang B, et al. Molecular switch role of Akt in Polygonatum odoratum lectin-induced apoptosis and autophagy in human non-small cell lung cancer A549 cells. PLoS ONE. 2014;9:e101526 pubmed publisher
Chiang E, Tsai S, Kuo Y, Pai M, Chiu H, Rodriguez R, et al. Caffeic acid derivatives inhibit the growth of colon cancer: involvement of the PI3-K/Akt and AMPK signaling pathways. PLoS ONE. 2014;9:e99631 pubmed publisher
Sahlberg S, Spiegelberg D, Glimelius B, Stenerlow B, Nestor M. Evaluation of cancer stem cell markers CD133, CD44, CD24: association with AKT isoforms and radiation resistance in colon cancer cells. PLoS ONE. 2014;9:e94621 pubmed publisher
Cam M, Bid H, Xiao L, Zambetti G, Houghton P, Cam H. p53/TAp63 and AKT regulate mammalian target of rapamycin complex 1 (mTORC1) signaling through two independent parallel pathways in the presence of DNA damage. J Biol Chem. 2014;289:4083-94 pubmed publisher
Bhalla S, Evens A, Prachand S, Schumacker P, Gordon L. Paradoxical regulation of hypoxia inducible factor-1? (HIF-1?) by histone deacetylase inhibitor in diffuse large B-cell lymphoma. PLoS ONE. 2013;8:e81333 pubmed publisher
Elloul S, Kedrin D, Knoblauch N, Beck A, Toker A. The adherens junction protein afadin is an AKT substrate that regulates breast cancer cell migration. Mol Cancer Res. 2014;12:464-76 pubmed publisher
Shukla S, Pia Patric I, Thinagararjan S, Srinivasan S, Mondal B, Hegde A, et al. A DNA methylation prognostic signature of glioblastoma: identification of NPTX2-PTEN-NF-?B nexus. Cancer Res. 2013;73:6563-73 pubmed publisher
Cheng Z, Gong Y, Ma Y, Lu K, Lu X, Pierce L, et al. Inhibition of BET bromodomain targets genetically diverse glioblastoma. Clin Cancer Res. 2013;19:1748-59 pubmed publisher
Pande S, Browne G, Padmanabhan S, Zaidi S, Lian J, Van Wijnen A, et al. Oncogenic cooperation between PI3K/Akt signaling and transcription factor Runx2 promotes the invasive properties of metastatic breast cancer cells. J Cell Physiol. 2013;228:1784-92 pubmed publisher
Ramaswamy S, Nakamura N, Vazquez F, Batt D, Perera S, Roberts T, et al. Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci U S A. 1999;96:2110-5 pubmed
product information
Catalog Number :
9008
Product Name :
1036 pcDNA3 Myr HA Akt1
article :
doi10.1073/pnas.96.5.2110
id351
pubmed_id10051603
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The sequence of the HA tag in this plasmid is YAYDVPDYA instead of YPYDVPDYA. Not all HA antibodies will recognize this sequence. Cell Signaling Technology's HA antibody #2367 will work.
growth strain :
Mammalian expression of human Akt1 with myr, HA tags
origin :
37
pi :
alt_names
Akt
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
mutation
nameAkt1
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmyr
locationN terminal on backbone
tagHA
resistance markers :
455
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA