904 pLNCX HA Akt1 K179M | Addgene 9007 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

904 pLNCX HA Akt1 K179M

catalog :

9007

citations: 4

Reference
Hamilton L, Dufresne M, JoppÃ© S, Petryszyn S, Aumont A, Calon F, et al. Aberrant Lipid Metabolism in the Forebrain Niche Suppresses Adult Neural Stem Cell Proliferation in an Animal Model of Alzheimer's Disease. Cell Stem Cell. 2015;17:397-411 pubmed publisher
Zeng M, van der Donk W, Chen J. Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt. Mol Biol Cell. 2014;25:3954-61 pubmed publisher
Wang X, Zhang H, Abel A, Young A, Xie L, Xie Z. Role of phosphatidylinositol 3-kinase (PI3K) and Akt1 kinase in porcine reproductive and respiratory syndrome virus (PRRSV) replication. Arch Virol. 2014;159:2091-6 pubmed publisher
Ramaswamy S, Nakamura N, Vazquez F, Batt D, Perera S, Roberts T, et al. Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci U S A. 1999;96:2110-5 pubmed

product information

Catalog Number :

9007

Product Name :

904 pLNCX HA Akt1 K179M

article :

doi	10.1073/pnas.96.5.2110
id	351
pubmed_id	10051603

bacterial resistance :

Ampicillin

cloning :

backbone

pLNCX

backbone_mutation

backbone_origin

BD Biosciences

backbone_size

6600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

An Akt-1 cDNA was amplified by reverse transcription-coupled PCR from total HeLa cell RNA and reamplified with a 5' primer containing a Kozak sequence and sequences encoding a hemagglutinin (HA) epitope and cloned into pLNCX to give pLNCX-HA-Akt. K179M mutation generated by site-directed mutagenesis.

origin :

pi :

alt_names

Akt1

Akt

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	CMV Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
gene	AKT1
id	207

genbank_ids

mutation

K179M. Kinase dead.

name

Akt1 K179M

shRNA_sequence

size

1500

species

9606

Homo sapiens