902 pLNCX myr HA Akt1 K179M | Addgene 9006 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

902 pLNCX myr HA Akt1 K179M

catalog :

9006

citations: 3

Reference
Gwinn D, Lee A, Briones Martin del Campo M, Conn C, Simpson D, Scott A, et al. Oncogenic KRAS Regulates Amino Acid Homeostasis and Asparagine Biosynthesis via ATF4 and Alters Sensitivity to L-Asparaginase. Cancer Cell. 2018;33:91-107.e6 pubmed publisher
Wasson C, Morgan E, Müller M, Ross R, Hartley M, Roberts S, et al. Human papillomavirus type 18 E5 oncogene supports cell cycle progression and impairs epithelial differentiation by modulating growth factor receptor signalling during the virus life cycle. Oncotarget. 2017;8:103581-103600 pubmed publisher
Ramaswamy S, Nakamura N, Vazquez F, Batt D, Perera S, Roberts T, et al. Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci U S A. 1999;96:2110-5 pubmed

product information

Catalog Number :

9006

Product Name :

902 pLNCX myr HA Akt1 K179M

article :

doi	10.1073/pnas.96.5.2110
id	351
pubmed_id	10051603

bacterial resistance :

Ampicillin

cloning :

backbone

pLNCX

backbone_mutation

backbone_origin

BD Biosciences

backbone_size

6600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

An Akt-1 cDNA was amplified by reverse transcription-coupled PCR from total HeLa cell RNA and reamplified with a 5' primer containing a Kozak sequence and sequences encoding a hemagglutinin (HA) epitope and cloned into pLNCX to give pLNCX-HA-Akt. A double-stranded oligonucleotide encoding the src myristoylation sequence was inserted 5' of the HA tag to generate pLNCX-Myr-HA-Akt. K179M mutation generated using site-directed mutagenesis.

origin :

pi :

alt_names

Akt1

Akt

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	CMV Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
gene	AKT1
id	207

genbank_ids

mutation

K179M. kinase dead.

name

Akt1 K179M

shRNA_sequence

size

1500

species

9606

Homo sapiens