901 pLNCX myr HA Akt1 | Addgene 9005 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

901 pLNCX myr HA Akt1

catalog :

9005

citations: 17

Reference
Mohapatra P, Mohanty S, Ansari S, Shriwas O, Ghosh A, Rath R, et al. CMTM6 attenuates cisplatin-induced cell death in OSCC by regulating AKT/c-Myc-driven ribosome biogenesis. FASEB J. 2022;36:e22566 pubmed publisher
Singh D, Mohapatra P, Kumar S, Behera S, Dixit A, Sahoo S. Nimbolide-encapsulated PLGA nanoparticles induces Mesenchymal-to-Epithelial Transition by dual inhibition of AKT and mTOR in pancreatic cancer stem cells. Toxicol In Vitro. 2022;79:105293 pubmed publisher
Walia V, Cuenca A, Vetter M, Insinna C, Perera S, Lu Q, et al. Akt Regulates a Rab11-Effector Switch Required for Ciliogenesis. Dev Cell. 2019;: pubmed publisher
Högstrand K, Darmanin S, Forshell T, Grandien A. Transformation of mouse T cells requires MYC and AKT activity in conjunction with inhibition of intrinsic apoptosis. Oncotarget. 2018;9:21396-21410 pubmed publisher
Gwinn D, Lee A, Briones Martin del Campo M, Conn C, Simpson D, Scott A, et al. Oncogenic KRAS Regulates Amino Acid Homeostasis and Asparagine Biosynthesis via ATF4 and Alters Sensitivity to L-Asparaginase. Cancer Cell. 2018;33:91-107.e6 pubmed publisher
Wasson C, Morgan E, Müller M, Ross R, Hartley M, Roberts S, et al. Human papillomavirus type 18 E5 oncogene supports cell cycle progression and impairs epithelial differentiation by modulating growth factor receptor signalling during the virus life cycle. Oncotarget. 2017;8:103581-103600 pubmed publisher
Bosch VilarÃ³ A, Jacobs B, Pomella V, Abbasi Asbagh L, Kirkland R, Michel J, et al. Feedback activation of HER3 attenuates response to EGFR inhibitors in colon cancer cells. Oncotarget. 2017;8:4277-4288 pubmed publisher
Shao J, Yang X, Liu T, Zhang T, Xie Q, Xia W. Autophagy induction by SIRT6 is involved in oxidative stress-induced neuronal damage. Protein Cell. 2016;7:281-290 pubmed publisher
Yamaguchi K, Takanashi T, Nasu K, Tamai K, Mochizuki M, Satoh I, et al. Xenotransplantation elicits salient tumorigenicity of adult T-cell leukemia-derived cells via aberrant AKT activation. Cancer Sci. 2016;107:638-43 pubmed publisher
Carrick B, Hao L, Smaldino P, Engelke D. A Novel Recombinant DNA System for High Efficiency Affinity Purification of Proteins in Saccharomyces cerevisiae. G3 (Bethesda). 2015;6:573-8 pubmed publisher
Quan Y, Wang N, Chen Q, Xu J, Cheng W, Di M, et al. SIRT3 inhibits prostate cancer by destabilizing oncoprotein c-MYC through regulation of the PI3K/Akt pathway. Oncotarget. 2015;6:26494-507 pubmed publisher
Wu Y, Deng Z, Tang Y, Zhang S, Zhang Y. Over-expressing Akt in T cells to resist tumor immunosuppression and increase anti-tumor activity. BMC Cancer. 2015;15:603 pubmed publisher
Saeed M, Andreo U, Chung H, Espiritu C, Branch A, Silva J, et al. SEC14L2 enables pan-genotype HCV replication in cell culture. Nature. 2015;524:471-5 pubmed publisher
Wang X, Zhang H, Abel A, Young A, Xie L, Xie Z. Role of phosphatidylinositol 3-kinase (PI3K) and Akt1 kinase in porcine reproductive and respiratory syndrome virus (PRRSV) replication. Arch Virol. 2014;159:2091-6 pubmed publisher
Ivanov S, Roy C. Pathogen signatures activate a ubiquitination pathway that modulates the function of the metabolic checkpoint kinase mTOR. Nat Immunol. 2013;14:1219-28 pubmed publisher
Lu H, Wang X, Urvalek A, Li T, Xie H, Yu L, et al. Transformation of human ovarian surface epithelial cells by Krüppel-like factor 8. Oncogene. 2014;33:10-8 pubmed publisher
Ramaswamy S, Nakamura N, Vazquez F, Batt D, Perera S, Roberts T, et al. Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci U S A. 1999;96:2110-5 pubmed

product information

Catalog Number :

9005

Product Name :

901 pLNCX myr HA Akt1

article :

doi	10.1073/pnas.96.5.2110
id	351
pubmed_id	10051603

bacterial resistance :

Ampicillin

cloning :

backbone

pLNCX

backbone_mutation

backbone_origin

BD Biosciences

backbone_size

6600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

An Akt-1 cDNA was amplified by reverse transcription-coupled PCR from total HeLa cell RNA and reamplified with a 5' primer containing a Kozak sequence and sequences encoding a hemagglutinin (HA) epitope and cloned into pLNCX to give pLNCX-HA-Akt. A double-stranded oligonucleotide encoding the src myristoylation sequence was inserted 5' of the HA tag to generate pLNCX-Myr-HA-Akt.

origin :

pi :

alt_names

Akt

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	CMV Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
gene	AKT1
id	207

genbank_ids

mutation

name

Akt1

shRNA_sequence

size

1500

species

9606

Homo sapiens