This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
903 pLNCX HA Akt1
catalog :
9004
citations: 5
Reference
Gwinn D, Lee A, Briones Martin del Campo M, Conn C, Simpson D, Scott A, et al. Oncogenic KRAS Regulates Amino Acid Homeostasis and Asparagine Biosynthesis via ATF4 and Alters Sensitivity to L-Asparaginase. Cancer Cell. 2018;33:91-107.e6 pubmed publisher
Wu Y, Deng Z, Tang Y, Zhang S, Zhang Y. Over-expressing Akt in T cells to resist tumor immunosuppression and increase anti-tumor activity. BMC Cancer. 2015;15:603 pubmed publisher
Vivanco I, Chen Z, Tanos B, Oldrini B, Hsieh W, Yannuzzi N, et al. A kinase-independent function of AKT promotes cancer cell survival. elife. 2014;3: pubmed publisher
Wang X, Zhang H, Abel A, Young A, Xie L, Xie Z. Role of phosphatidylinositol 3-kinase (PI3K) and Akt1 kinase in porcine reproductive and respiratory syndrome virus (PRRSV) replication. Arch Virol. 2014;159:2091-6 pubmed publisher
Ramaswamy S, Nakamura N, Vazquez F, Batt D, Perera S, Roberts T, et al. Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci U S A. 1999;96:2110-5 pubmed
product information
Catalog Number :
9004
Product Name :
903 pLNCX HA Akt1
article :
doi10.1073/pnas.96.5.2110
id351
pubmed_id10051603
bacterial resistance :
Ampicillin
cloning :
backbonepLNCX
backbone_mutation
backbone_originBD Biosciences
backbone_size6600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
An Akt-1 cDNA was amplified by reverse transcription-coupled PCR from total HeLa cell RNA and reamplified with a 5' primer containing a Kozak sequence and sequences encoding a hemagglutinin (HA) epitope and cloned into pLNCX to give pLNCX-HA-Akt.
origin :
37
pi :
alt_names
Akt
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
mutation
nameAkt1
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
455
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA