This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
896 pcDNA3 T7 Akt1
catalog :
9003
citations: 5
Reference
Jeong M, Jeong M, Kim J, Cho S, Lee K, Park S, et al. TCTP protein degradation by targeting mTORC1 and signaling through S6K, Akt, and Plk1 sensitizes lung cancer cells to DNA-damaging drugs. Sci Rep. 2021;11:20812 pubmed publisher
Bjune K, Sundvold H, Leren T, Naderi S. MK-2206, an allosteric inhibitor of AKT, stimulates LDLR expression and LDL uptake: A potential hypocholesterolemic agent. Atherosclerosis. 2018;276:28-38 pubmed publisher
Gong L, Di C, Xia X, Wang J, Chen G, Shi J, et al. AKT/mTOR signaling pathway is involved in salvianolic acid B-induced autophagy and apoptosis in hepatocellular carcinoma cells. Int J Oncol. 2016;49:2538-2548 pubmed publisher
Kolbe K, Bukhari H, Loosse C, Leonhardt G, Glotzbach A, Pawlas M, et al. Extensive nuclear sphere generation in the human Alzheimer's brain. Neurobiol Aging. 2016;48:103-113 pubmed publisher
Ramaswamy S, Nakamura N, Vazquez F, Batt D, Perera S, Roberts T, et al. Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci U S A. 1999;96:2110-5 pubmed
product information
Catalog Number :
9003
Product Name :
896 pcDNA3 T7 Akt1
article :
doi10.1073/pnas.96.5.2110
id351
pubmed_id10051603
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Created by Francisca Vazquez. A cDNA for AKT-1 was PCR-amplified from a fetal brain library and ligated to the vector from pCDNA3-T7-VHL to give pCDNA3-T7-AKT.
origin :
37
pi :
alt_names
Akt
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
mutation
nameAkt1
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
resistance markers :
455
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA