This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro
catalog :
89992
citations: 1
Reference
Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T. Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9. Stem Cell Res. 2017;23:105-108 pubmed publisher
product information
Catalog Number :
89992
Product Name :
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro
article :
doi10.1016/j.scr.2017.07.006
id28191936
pubmed_id28925359
bacterial resistance :
Ampicillin
cloning :
backbonepUC19
backbone_mutation
backbone_origin
backbone_size2647
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth strain :
Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5XhoI
promoternone
sequencing_primer_3M13-rv caggaaacagctatgaccatg
sequencing_primer_5M13-fwd tgtaaaacgacggccagt
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
genbank_ids
mutation
nameOCT4-T2A-NLS-EmGFP-P2A-Puro
shRNA_sequence
size2250
species
9606
Homo sapiens
tags
plasmid copy :
tdTomato sequence was cloned from pCSCMV:tdTomato (a gift from Gerhart Ryffel, Addgene plasmid #30530)
resistance markers :
2561
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA