This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro
catalog :
89991
citations: 2
Reference
Lee M, Choi N, Park S, Bang J, Lee Y, Jeong D, et al. Generation of OCT4-EGFP, NANOG-tdTomato dual reporter human induced pluripotent stem cell line, KKUi001-A, using the CRISPR/Cas9 system. Stem Cell Res. 2020;48:101943 pubmed publisher
Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T. Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9. Stem Cell Res. 2017;22:16-19 pubmed publisher
product information
Catalog Number :
89991
Product Name :
pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro
article :
doi10.1016/j.scr.2017.05.005
id28191984
pubmed_id28952927
bacterial resistance :
Ampicillin
cloning :
backbonepUC19
backbone_mutation
backbone_origin
backbone_size2657
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth strain :
Donor template for generation of SOX2-ntdTomato reporter cell lines
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BamHI
promoternone
sequencing_primer_3M13-rv caggaaacagctatgaccatg
sequencing_primer_5M13-fwd tgtaaaacgacggccagt
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
genbank_ids
mutation
nameSOX2-T2A-2xNLS-TdTomato-F2A-Puro
shRNA_sequence
size3667
species
9606
Homo sapiens
tags
plasmid copy :
TdTomato sequence was cloned from pCSCMV:tdTomato (a gift from Gerhart Ryffel, Addgene plasmid #30530).
resistance markers :
2561
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA