This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV MN (eGFP)
catalog :
89932
citations: 1
Reference
Xiong T, Meister G, Workman R, Kato N, Spellberg M, Turker F, et al. Targeted DNA methylation in human cells using engineered dCas9-methyltransferases. Sci Rep. 2017;7:6732 pubmed publisher
product information
Catalog Number :
89932
Product Name :
pCMV MN (eGFP)
article :
doi10.1038/s41598-017-06757-0 [pii]
id28191826
pubmed_id28751638
bacterial resistance :
Ampicillin
cloning :
backbonepCMV
backbone_mutation
backbone_origin
backbone_size4156
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Does not contain active methylatransferase - general cloning strains can be used.
growth strain :
Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.
origin :
37
pi :
alt_names
NLS-HA-MN
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3gcttgccaaacctacaggtgg
sequencing_primer_5CGTGTACGGTGGGAGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
X17195
mutationM.SssI residues 2-272 fragment
nameMN
shRNA_sequence
size867
species
tags
locationN terminal on insert
tagHA
resistance markers :
366
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA