This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV sMTase (eGFP)
catalog :
89930
citations: 1
Reference
Xiong T, Meister G, Workman R, Kato N, Spellberg M, Turker F, et al. Targeted DNA methylation in human cells using engineered dCas9-methyltransferases. Sci Rep. 2017;7:6732 pubmed publisher
product information
Catalog Number :
89930
Product Name :
pCMV sMTase (eGFP)
article :
doi10.1038/s41598-017-06757-0 [pii]
id28191826
pubmed_id28751638
bacterial resistance :
Ampicillin
cloning :
backbonepCMV
backbone_mutation
backbone_origin
backbone_size4156
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.
growth temp :
Requires strain that is tolerant of CpG methylation
inserts :
NEB 10-beta or ER2267
origin :
37
pi :
alt_names
NLS-Flag-dCas9-lfl-M.SssI[273-386]-NLS - IRES - NLS-HA-M.SssI[2-272]
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3gcttgccaaacctacaggtgg
sequencing_primer_5CGTGTACGGTGGGAGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationdeactivated Cas9
namedCas9-MC-IRES-MN
shRNA_sequence
size6156
species
tags
locationN terminal on insert
tagFlag
locationN terminal on insert
tagHA
resistance markers :
366
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA