This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMT2 Alpha2 delta-1 V6
catalog :
89894
citations: 1
Reference
Kadurin I, Ferron L, Rothwell S, Meyer J, Douglas L, Bauer C, et al. Proteolytic maturation of ?2? represents a checkpoint for activation and neuronal trafficking of latent calcium channels. elife. 2016;5: pubmed publisher
product information
Catalog Number :
89894
Product Name :
pMT2 Alpha2 delta-1 V6
article :
doi10.7554/eLife.21143
id25358
pubmed_id27782881
bacterial resistance :
Ampicillin
cloning :
backbonepMT2
backbone_mutation
backbone_origin
backbone_size5129
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
5 amino acids are mutated to valines in the region believe to be involved in post translational cleavage of the alpha2 and delta-1 moities (there is already a Valine at AA position 946) leading to a stretch of 6 valines
growth strain :
Mammalian expression of Alpha2 delta-1 V6
origin :
37
pi :
alt_names
Alpha2delta-1
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5KpnI
promoteradenovirus major late promoter
sequencing_primer_35' ggtcgaaccatgatggcagc 3'
sequencing_primer_55' agcttgaggtgtggcaggctt 3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCHLA2, Cacna2, DHSCCA
geneCacna2d1
id25399
genbank_ids
mutationAmino acids L943V, E944V, A945V, E947V, M948V are mutated.
nameCacna2d1
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
resistance markers :
2017
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA