pMT2 Beta1b mCherry | Addgene 89892 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMT2 Beta1b mCherry

catalog :

89892

citations: 1

Reference
Page K, Rothwell S, Dolphin A. The CaV? Subunit Protects the I-II Loop of the Voltage-gated Calcium Channel CaV2.2 from Proteasomal Degradation but Not Oligoubiquitination. J Biol Chem. 2016;291:20402-16 pubmed publisher

product information

Catalog Number :

89892

Product Name :

pMT2 Beta1b mCherry

article :

doi	10.1074/jbc.M116.737270
id	25356
pubmed_id	27489103

bacterial resistance :

Ampicillin

cloning :

backbone

pMT2

backbone_mutation

backbone_origin

backbone_size

5129

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Cut out B1b with EcoRI and SpeI (but then you lose the start codon), cut out mCherry with SpeI and NotI (but then you lose the Stop codon). The mCherry is missing the N-terminal start codon. The SpeI site introduces 2 extra amino acids: Threonine and serine. Cut out the entire insert with EcoRI and NotI.

growth strain :

Mammalian expression of Beta1b mCherry

origin :

pi :

alt_names

Beta1b

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	EcoRI
promoter	adenovirus major late promoter
sequencing_primer_3	5' ggtcgaaccatgatggcagc 3'
sequencing_primer_5	5' agcttgaggtgtggcaggctt 3'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Cacnb1
id	50688

genbank_ids

mutation

mCherry sequence inserted just before the Stop codon

name

Cacnb1

shRNA_sequence

size

2658

species

10116

Rattus norvegicus