This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMT2 Cav2.2 DomI and DomII
catalog :
89891
citations: 1
Reference
Raghib A, Bertaso F, Davies A, Page K, Meir A, Bogdanov Y, et al. Dominant-negative synthesis suppression of voltage-gated calcium channel Cav2.2 induced by truncated constructs. J Neurosci. 2001;21:8495-504 pubmed
product information
Catalog Number :
89891
Product Name :
pMT2 Cav2.2 DomI and DomII
article :
doi21/21/8495 [pii]
id8738
pubmed_id11606638
bacterial resistance :
Ampicillin
cloning :
backbonepMT2
backbone_mutation
backbone_origin
backbone_size5129
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Contains Cav2.2 N-terminus, Domain I, the I-II loop, Domain II and the II-III loop.
growth strain :
Mammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2
origin :
37
pi :
alt_names
Alpha1B
cloning
clone_methodRestriction Enzyme
cloning_site_3SpeI
cloning_site_5EcoRI
promoteradenovirus major late promoter
sequencing_primer_35' ggtcgaaccatgatggcagc 3'
sequencing_primer_55' agcttgaggtgtggcaggctt 3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCACNA1B
id100008979
genbank_ids
mutationStop codon after amino acid 1154 (this is a truncated form of the wild type gene)
nameCacna1b
shRNA_sequence
size3505
species
32644
Other
tags
resistance markers :
2017
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA