pcDNA3-myc-FLNa S2152A | Addgene 8983 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3-myc-FLNa S2152A

catalog :

8983

citations: 6

Reference
Urra H, Henríquez D, Cánovas J, Villarroel Campos D, Carreras Sureda A, Pulgar E, et al. IRE1α governs cytoskeleton remodelling and cell migration through a direct interaction with filamin A. Nat Cell Biol. 2018;20:942-953 pubmed publisher
van Vliet A, Giordano F, Gerlo S, Segura I, Van Eygen S, Molenberghs G, et al. The ER Stress Sensor PERK Coordinates ER-Plasma Membrane Contact Site Formation through Interaction with Filamin-A and F-Actin Remodeling. Mol Cell. 2017;65:885-899.e6 pubmed publisher
Sousa Ã, Pereira P, Sousa F, Queiroz J. Binding mechanisms for histamine and agmatine ligands in plasmid deoxyribonucleic acid purifications. J Chromatogr A. 2014;1366:110-9 pubmed publisher
Bicho D, Sousa A, Sousa F, Queiroz J, Tomaz C. Effect of chromatographic conditions and plasmid DNA size on the dynamic binding capacity of a monolithic support. J Sep Sci. 2014;37:2284-92 pubmed publisher
Venkatareddy M, Cook L, Abuarquob K, Verma R, Garg P. Nephrin regulates lamellipodia formation by assembling a protein complex that includes Ship2, filamin and lamellipodin. PLoS ONE. 2011;6:e28710 pubmed publisher
Woo M, Ohta Y, Rabinovitz I, Stossel T, Blenis J. Ribosomal S6 kinase (RSK) regulates phosphorylation of filamin A on an important regulatory site. Mol Cell Biol. 2004;24:3025-35 pubmed

product information

Catalog Number :

8983

Product Name :

pcDNA3-myc-FLNa S2152A

article :

doi	10.1128/MCB.24.7.3025-3035.2004
id	343
pubmed_id	15024089

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

backbone_size

5446

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

S2152A mutation made using Stratgene Quikchange mutagenesis kit. 5' FLNa-Ala2152 primer (GCAGGCGTCGGGCTCCTGCAGTGGCCAACGTTG) and 3' FLNa-Ala2152 primer (CAACGTTGGCCACTGCAGGAGCCCGACGCCTGC). See author's map for details of wild-type version of this plasmid.

origin :

pi :

alt_names

Filamin A

FLNa

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	HindIII
promoter
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ABP-280, ABPX, CSBS, CVD1, FGS2, FLN, FLN-A, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD
gene	FLNA
id	2316

genbank_ids

mutation

Ser2152Ala

name

Filamin A S2152A

shRNA_sequence

size

8640

species

9606

Homo sapiens