This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Rc/CMV cyclin E
catalog :
8963
citations: 6
Reference
Krachmarova E, Petkov P, Lilkova E, Ilieva N, Rangelov M, Todorova N, et al. Insights into the SARS-CoV-2 ORF6 Mechanism of Action. Int J Mol Sci. 2023;24: pubmed publisher
Ferraiuolo R, Fifield B, Hamm C, Porter L. Stabilization of c-Myc by the atypical cell cycle regulator, Spy1, decreases efficacy of breast cancer treatments. Breast Cancer Res Treat. 2022;196:17-30 pubmed publisher
Kok Y, Guerrero Llobet S, Schoonen P, Everts M, Bhattacharya A, Fehrmann R, et al. Overexpression of Cyclin E1 or Cdc25A leads to replication stress, mitotic aberrancies, and increased sensitivity to replication checkpoint inhibitors. Oncogenesis. 2020;9:88 pubmed publisher
Matson J, Dumitru R, Coryell P, Baxley R, Chen W, Twaroski K, et al. Rapid DNA replication origin licensing protects stem cell pluripotency. elife. 2017;6: pubmed publisher
Ferraiuolo R, Tubman J, Sinha I, Hamm C, Porter L. The cyclin-like protein, SPY1, regulates the ER? and ERK1/2 pathways promoting tamoxifen resistance. Oncotarget. 2017;8:23337-23352 pubmed publisher
Hinds P, Mittnacht S, Dulic V, Arnold A, Reed S, Weinberg R. Regulation of retinoblastoma protein functions by ectopic expression of human cyclins. Cell. 1992;70:993-1006 pubmed
product information
Catalog Number :
8963
Product Name :
Rc/CMV cyclin E
article :
doi10.1016/0092-8674(92)90249-C
id339
pubmed_id1388095
bacterial resistance :
Ampicillin
cloning :
backbonepRc/CMV
backbone_mutation
backbone_origin
backbone_size5542
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
See author's map. Please note BstXI was used to clone in insert (blunt ligation). There are also 2 EcoRI sites flanking the insert.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BstXI
cloning_site_5BstXI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCCNE, pCCNE1
geneCCNE1
id898
genbank_ids
NM_001322262.1
mutation
namecyclin E
shRNA_sequence
size2500
species
9606
Homo sapiens
tags
locationC terminal on insert
tagGPI linker
resistance markers :
568
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA