Rc/CMV cyclin D1 HA | Addgene 8948 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Rc/CMV cyclin D1 HA

catalog :

8948

citations: 4

Reference
Kolapalli S, Sahu R, Chauhan N, Jena K, Mehto S, Das S, et al. RNA-Binding RING E3-Ligase DZIP3/hRUL138 Stabilizes Cyclin D1 to Drive Cell-Cycle and Cancer Progression. Cancer Res. 2021;81:315-331 pubmed publisher
Mohanty A, Sandoval N, Das M, Pillai R, Chen L, Chen R, et al. CCND1 mutations increase protein stability and promote ibrutinib resistance in mantle cell lymphoma. Oncotarget. 2016;7:73558-73572 pubmed publisher
Hwang S, Lee H, Kim H, Song H, Lee D, Lee H, et al. Overexpression of microRNA-95-3p suppresses brain metastasis of lung adenocarcinoma through downregulation of cyclin D1. Oncotarget. 2015;6:20434-48 pubmed
Baker G, Landis M, Hinds P. Multiple functions of D-type cyclins can antagonize pRb-mediated suppression of proliferation. Cell Cycle. 2005;4:330-8 pubmed

product information

Catalog Number :

8948

Product Name :

Rc/CMV cyclin D1 HA

article :

doi	10.4161/cc.4.2.1485
id	337
pubmed_id	15684604

bacterial resistance :

Ampicillin

cloning :

backbone

pRc/CMV

backbone_mutation

backbone_origin

backbone_size

5542

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A PCR product was digested with StuI and BspEI and the resulting ~250 bp fragment was used to replace the corresponding wildtype fragment. This resulted in the addition of the HA epitope after the final amino acid of cyclin D1 and reiteration of the final two cyclind D1 residues (DI) after the tag. In addition, 80 bp of the 3' UTS is deleted. For parent plasmid information, see Author's Map link for Rc/CMV cyclin D1.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	HindIII
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCL1, D11S287E, PRAD1, U21B31
gene	CCND1
id	595

genbank_ids

mutation

name

cyclin D1

shRNA_sequence

size

1100

species

9606

Homo sapiens