pMarC9-R6k | Addgene 89477 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMarC9-R6k

catalog :

89477

citations: 2

Reference
Su Y, Xu Y, Li Q, Yuan G, Zheng D. The essential genome of Ralstonia solanacearum. Microbiol Res. 2020;238:126500 pubmed publisher
Lee H, Ostrov N, Wong B, Gold M, Khalil A, Church G. Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi. Nat Microbiol. 2019;4:1105-1113 pubmed publisher

product information

Catalog Number :

89477

Product Name :

pMarC9-R6k

article :

doi	10.1038/s41564-019-0423-8
id	25247
pubmed_id	30962569

bacterial resistance :

Ampicillin and Kanamycin

cloning :

backbone	pBAM1
backbone_mutation	Tn5 replaced with marC9. Mariner transposon ends cloned into the transposon cassette. The transposon ends are mutagenized to introduce an mmeI cut site.
backbone_origin	Victor de Lorenzo lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

BW29427 is an E. coli strain with diaminopimelic acid (DAP) auxotrophy. BW29427 growth requires 300uM DAP even when cultured in LB. Importantly, BW29427 does not grow in the absence of DAP, which simplifies counterselection of this host strain following biparental mating with Vibrio natriegens. Please visit https://www.biorxiv.org/content/early/2016/06/12/058487 for bioRxiv preprint.

growth strain :

transposon plasmid, compatible with Tn-Seq

growth temp :

Use BW29427 strain supplemented with 300uM DAP for this plasmid when conjugating from E. coli to Vibrio natriegens

origin :

pi :

alt_names
cloning
entrez_gene
genbank_ids
mutation
name	pMarC9-R6k
shRNA_sequence
size
species
tags

resistance markers :

765

tags :

Unknown