pTetON-DHFRdd-SpvB; CMV-mCherry | Addgene 89463 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTetON-DHFRdd-SpvB; CMV-mCherry

catalog :

89463

citations: 3

Reference
Tello Lafoz M, Srpan K, Sanchez E, Hu J, Remšík J, Romin Y, et al. Cytotoxic lymphocytes target characteristic biophysical vulnerabilities in cancer. Immunity. 2021;54:1037-1054.e7 pubmed publisher
Schätzle P, Esteves da Silva M, Tas R, Katrukha E, Hu H, Wierenga C, et al. Activity-Dependent Actin Remodeling at the Base of Dendritic Spines Promotes Microtubule Entry. Curr Biol. 2018;28:2081-2093.e6 pubmed publisher
Harterink M, da Silva M, Will L, Turan J, Ibrahim A, Lang A, et al. DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells. Nat Methods. 2017;14:479-482 pubmed publisher

product information

Catalog Number :

89463

Product Name :

pTetON-DHFRdd-SpvB; CMV-mCherry

article :

doi	10.1038/nmeth.4257
id	25519
pubmed_id	28394337

bacterial resistance :

Ampicillin

cloning :

backbone

pTRE3G-luciferase

backbone_mutation

Removed luciferase, replaced with EGFP

backbone_origin

Clontech

backbone_size

3383

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Uses Clontech 3rd generation Tet-responsive promoter; also requires Tet-On 3G transactivator protein. E. coli dihydrofolate reductase destabilization domain (DHFRdd) with R12Y, G67S, Y100I mutations was a kind gift of Tom Wandless (Stanford): Iwamoto, M., Bj rklund, T., Lundberg, C., Kirik, D. & Wandless, T. J. A general chemical method to regulate protein stability in the mammalian central nervous system. Chem. Biol. 17, 981 988 (2010).

growth strain :

Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)

origin :

pi :

alt_names

Salmonella SpvB(375-591)

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	TRE3G
sequencing_primer_3	CATCAGTTCCATAGGTTG
sequencing_primer_5	gcagagctcgtttagtgaa
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	pOU1113_03
gene	spvB
id	3523601

genbank_ids

D14490.1

mutation

Truncation spanning SpvB amino acids 375-591 (mono(ADP-ribosyl)transferase domain)

name

DeAct-SpvB

shRNA_sequence

size

648

species

32644

Other

Salmonella enterica