This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTetON-DeAct-SpvB
catalog :
89455
citations: 2
Reference
Vilches Barro A, Stöckle D, Thellmann M, Ruiz Duarte P, Bald L, Louveaux M, et al. Cytoskeleton Dynamics Are Necessary for Early Events of Lateral Root Initiation in Arabidopsis. Curr Biol. 2019;29:2443-2454.e5 pubmed publisher
Harterink M, da Silva M, Will L, Turan J, Ibrahim A, Lang A, et al. DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells. Nat Methods. 2017;14:479-482 pubmed publisher
product information
Catalog Number :
89455
Product Name :
pTetON-DeAct-SpvB
article :
doi10.1038/nmeth.4257
id25519
pubmed_id28394337
bacterial resistance :
Ampicillin
cloning :
backbonepTRE3G-luciferase
backbone_mutationRemoved luciferase, replaced with EGFP-SpvB(375-591)
backbone_originClontech
backbone_size3383
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Uses Clontech 3rd generation Tet-responsive promoter; also requires Tet-On 3G transactivator protein
growth strain :
Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells
origin :
37
pi :
alt_names
Salmonella SpvB(375-591)
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterTRE3G
sequencing_primer_3CATCAGTTCCATAGGTTG
sequencing_primer_5gcagagctcgtttagtgaa
site_3_destroyed
site_5_destroyed
entrez_gene
aliasespOU1113_03
genespvB
id3523601
genbank_ids
D14490.1
mutationTruncation spanning SpvB amino acids 375-591 (mono(ADP-ribosyl)transferase domain)
nameDeAct-SpvB
shRNA_sequence
size648
species
32644
Other
Salmonella enterica
tags
locationN terminal on insert
tagEGFP
resistance markers :
3323
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA