pCMV-DeAct-SpvB | Addgene 89446 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV-DeAct-SpvB

catalog :

89446

citations: 3

Reference
Vilches Barro A, Stöckle D, Thellmann M, Ruiz Duarte P, Bald L, Louveaux M, et al. Cytoskeleton Dynamics Are Necessary for Early Events of Lateral Root Initiation in Arabidopsis. Curr Biol. 2019;29:2443-2454.e5 pubmed publisher
Schätzle P, Esteves da Silva M, Tas R, Katrukha E, Hu H, Wierenga C, et al. Activity-Dependent Actin Remodeling at the Base of Dendritic Spines Promotes Microtubule Entry. Curr Biol. 2018;28:2081-2093.e6 pubmed publisher
Harterink M, da Silva M, Will L, Turan J, Ibrahim A, Lang A, et al. DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells. Nat Methods. 2017;14:479-482 pubmed publisher

product information

Catalog Number :

89446

Product Name :

pCMV-DeAct-SpvB

article :

doi	10.1038/nmeth.4257
id	25519
pubmed_id	28394337

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

Clontech

backbone_size

4746

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

origin :

pi :

alt_names

Salmonella SpvB(375-591)

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	ATGTGGTATGGCTGATTATG
sequencing_primer_5	CATGGTCCTGCTGGAGTTCGTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	pOU1113_03
gene	spvB
id	3523601

genbank_ids

D14490.1

mutation

Truncation spanning SpvB amino acids 375-591 (mono(ADP-ribosyl)transferase domain)

name

DeAct-SpvB

shRNA_sequence

size

648

species

32644

Other

Salmonella enterica