article :
| doi | 10.1038/s41598-017-02693-1 [pii] |
| id | 28189709 |
| pubmed_id | 28550309 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pUC9 |
| backbone_mutation | |
| backbone_origin | |
| backbone_size | 2635 |
| promoter | |
| sequencing_primer_3 | |
| sequencing_primer_5 | |
| vector_types | |
growth strain :
High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders
pi :
| alt_names | | lambda DNA position 650-1149 |
| | cloning | | clone_method | Restriction Enzyme | | cloning_site_3 | None | | cloning_site_5 | HIndIII | | promoter | | | sequencing_primer_3 | N/A | | sequencing_primer_5 | N/A | | site_3_destroyed | | | site_5_destroyed | |
| | entrez_gene | | | genbank_ids | | | mutation | | | name | 500 bp EcoRV fragment | | shRNA_sequence | | | size | 500 | | species | | | tags | |
|
| alt_names | | lambda DNA position 6681-7681 |
| | cloning | | clone_method | Restriction Enzyme | | cloning_site_3 | None | | cloning_site_5 | None | | promoter | | | sequencing_primer_3 | N/A | | sequencing_primer_5 | N/A | | site_3_destroyed | | | site_5_destroyed | |
| | entrez_gene | | | genbank_ids | | | mutation | | | name | 1000 bp EcoRV fragment | | shRNA_sequence | | | size | 1000 | | species | | | tags | |
|
| alt_names | | lambda DNA position 28223-29710 |
| | cloning | | clone_method | Restriction Enzyme | | cloning_site_3 | EcoRI | | cloning_site_5 | None | | promoter | | | sequencing_primer_3 | N/A | | sequencing_primer_5 | N/A | | site_3_destroyed | | | site_5_destroyed | |
| | entrez_gene | | | genbank_ids | | | mutation | | | name | 1500 bp EcoRV fragment | | shRNA_sequence | | | size | 1500 | | species | | | tags | |
|
| alt_names | | lambda DNA position 36060-37994 |
| | cloning | | clone_method | Restriction Enzyme | | cloning_site_3 | BglII | | cloning_site_5 | XbaI | | promoter | | | sequencing_primer_3 | N/A | | sequencing_primer_5 | N/A | | site_3_destroyed | | | site_5_destroyed | |
| | entrez_gene | | | genbank_ids | | | mutation | | | name | 2000 bp EcoRV fragment | | shRNA_sequence | | | size | 2000 | | species | | | tags | |
|
| alt_names | | lambda DNA position 28223-28663 |
| | cloning | | | entrez_gene | | | genbank_ids | | | mutation | | | name | 500 bp PstI fragment | | shRNA_sequence | | | size | 500 | | species | | | tags | |
|
| alt_names | | lambda DNA position 8783-9468 |
| | cloning | | clone_method | Restriction Enzyme | | cloning_site_3 | BamHI | | cloning_site_5 | XbaI | | promoter | | | sequencing_primer_3 | N/A | | sequencing_primer_5 | N/A | | site_3_destroyed | 1 | | site_5_destroyed | |
| | entrez_gene | | | genbank_ids | | | mutation | | | name | 700 bp PstI fragment | | shRNA_sequence | | | size | 700 | | species | | | tags | |
|
| alt_names | | G9a position 2757-3535 | | EHMT2 |
| | cloning | | clone_method | Restriction Enzyme | | cloning_site_3 | BamHI | | cloning_site_5 | XbaI | | promoter | | | sequencing_primer_3 | N/A | | sequencing_primer_5 | N/A | | site_3_destroyed | 1 | | site_5_destroyed | |
| | entrez_gene | | aliases | BAT8, C6orf30, G9A, GAT8, KMT1C, NG36 | | gene | EHMT2 | | id | 10919 |
|
| | genbank_ids | | | mutation | | | name | 800 bp PstI fragment | | shRNA_sequence | | | size | 800 | | species | | | tags | |
|
| alt_names | | lambda DNA position 143381-15280 |
| | cloning | | clone_method | Restriction Enzyme | | cloning_site_3 | EcoRI | | cloning_site_5 | XbaI | | promoter | | | sequencing_primer_3 | N/A | | sequencing_primer_5 | N/A | | site_3_destroyed | | | site_5_destroyed | |
| | entrez_gene | | | genbank_ids | | | mutation | | | name | 900 bp PstI fragment | | shRNA_sequence | | | size | 900 | | species | | | tags | |
|
| alt_names | | | cloning | | | entrez_gene | | aliases | FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1 | | gene | BMI1 | | id | 648 |
|
| | genbank_ids | | | mutation | | | name | 1000 bp PstI fragment | | shRNA_sequence | | | size | 1000 | | species | | | tags | |
|
| alt_names | | lambda DNA position 28670-29710 and 36060-37994 |
| | cloning | | | entrez_gene | | | genbank_ids | | | mutation | | | name | 2000 bp PstI fragment | | shRNA_sequence | | | size | 2000 | | species | | | tags | |
|
