This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPS2972
catalog :
8942
citations: 1
Reference
Devroe E, Silver P. Retrovirus-delivered siRNA. BMC Biotechnol. 2002;2:15 pubmed
product information
Catalog Number :
8942
Product Name :
pPS2972
article :
doi10.1186/1472-6750-2-15
id329
pubmed_id12199908
bacterial resistance :
Ampicillin
cloning :
backbonepMSCVpuro
backbone_mutation
backbone_originClontech
backbone_size6300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
RNAi
growth notes :
siRNA targeting NDR1. BamHI-BamHI fragment from pBS/U6-NDR blunt-ended and inserted into pMSCVpuro blunt ended at the unique NsiI site. Small hairpin RNA corresponds to nucleotides 515-535 of NDR ORF (nucleotides 801-821 of NDR mRNA sequence, Genbank NM_007271).
origin :
37
pi :
alt_names
pMSCV/U6-NDR
siRNA NDR1
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesNDR, NDR1
geneSTK38
id11329
genbank_ids
NM_007271
mutation
nameNDR1
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
480
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA