pLL3.7m-psMEK1 | Addgene 89361 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLL3.7m-psMEK1

catalog :

89361

citations: 3

Reference
Patel A, Zhang L, Keenan S, RUSHLOW C, Fradin C, Shvartsman S. Capicua is a fast-acting transcriptional brake. Curr Biol. 2021;: pubmed publisher
Ariza A, Funahashi Y, Kozawa S, Omar Faruk M, Nagai T, Amano M, et al. Dynamic subcellular localization and transcription activity of the SRF cofactor MKL2 in the striatum are regulated by MAPK. J Neurochem. 2021;: pubmed publisher
Zhou X, Fan L, Li P, Shen K, Lin M. Optical control of cell signaling by single-chain photoswitchable kinases. Science. 2017;355:836-842 pubmed publisher

product information

Catalog Number :

89361

Product Name :

pLL3.7m-psMEK1

article :

doi	10.1126/science.aah3605
id	25216
pubmed_id	28232577

bacterial resistance :

Ampicillin

cloning :

backbone

pLL3.7m

backbone_mutation

5' and 3' UTRs of HIV transcript reduced while retaining essential packaging elements

backbone_origin

Luk Parijs

backbone_size

6500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth strain :

A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

origin :

pi :

alt_names

photoswitchable MEK1

photoswitchable MAPKK1

photoswitchable MAP2K1

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	CGGCCTTTTTACGGTTCCTG
sequencing_primer_5	CGGATAACAATTTCACACAG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
gene	MAP2K1
id	5604

genbank_ids

mutation

name

psMEK1

shRNA_sequence

size

species

9606

Homo sapiens

100

Synthetic