This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pOD1988-epiDEG
catalog :
89357
citations: 1
Reference
Wang S, Tang N, Lara Gonzalez P, Zhao Z, Cheerambathur D, Prevo B, et al. A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Development. 2017;144:2694-2701 pubmed publisher
product information
Catalog Number :
89357
Product Name :
pOD1988-epiDEG
article :
doi10.1242/dev.150094
id28189865
pubmed_id28619826
bacterial resistance :
Ampicillin
cloning :
backbonepCFJ151
backbone_mutation
backbone_originChristian Fr kj r-Jensen (Erik Jorgensen lab)
backbone_size7328
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Targeting vector for Mos1 transposon mediated single copy transgene insertion
growth strain :
MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterPdpy-7
sequencing_primer_3CGACTCACTAGTGGGCAGAT
sequencing_primer_5tgtaaaacgacggccagt
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_F59B2.6
genezif-1
id176274
genbank_ids
mutation
namevhhGFP4-ZIF-1
shRNA_sequence
size2098
species
6239
Caenorhabditis elegans
100
Synthetic
tags
locationN terminal on insert
tagvhhGFP4 (GFP nanobody)
resistance markers :
3319
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA