This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFS485
catalog :
89348
citations: 1
Reference
Keifenheim D, Sun X, D Souza E, Ohira M, Magner M, Mayhew M, et al. Size-Dependent Expression of the Mitotic Activator Cdc25 Suggests a Mechanism of Size Control in Fission Yeast. Curr Biol. 2017;27:1491-1497.e4 pubmed publisher
product information
Catalog Number :
89348
Product Name :
pFS485
article :
doi10.1016/j.cub.2017.04.016
id25211
pubmed_id28479325
bacterial resistance :
Ampicillin
cloning :
backbonepJK148
backbone_mutationcdc25 genomic fragment cloned into pJK148 with KpnI and SacI
backbone_originKeeney and Boeke, Genetics, 136:849 (1994)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
Leu1 integrating plasmid with cdc25 genomic fragment
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5SacI
promoter
sequencing_primer_3CATGGTCATAGCTGTTTCCTG
sequencing_primer_5TGTAAAACGACGGCCAGT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSPAC24H6.05, sal2
genecdc25
id2542632
genbank_ids
mutation
namecdc25
shRNA_sequence
size2804
species
4896
Schizosaccharomyces pombe
tags
resistance markers :
416
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA