This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta
catalog :
89347
citations: 2
Reference
Joung J, Kirchgatterer P, Singh A, Cho J, Nety S, Larson R, et al. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat Commun. 2022;13:1606 pubmed publisher
Taylor M, Husain K, Gartner Z, Mayor S, Vale R. A DNA-Based T Cell Receptor Reveals a Role for Receptor Clustering in Ligand Discrimination. Cell. 2017;169:108-119.e20 pubmed publisher
product information
Catalog Number :
89347
Product Name :
TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta
article :
doi10.1016/j.cell.2017.03.006
id28189728
pubmed_id28340336
bacterial resistance :
Ampicillin
cloning :
backbonepHR
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterSFFV
sequencing_primer_3WPRE rev (CCAGAGGTTGATTATCGATAAGC)
sequencing_primer_5SFFV for (GCTTCCCGAGCTCTATAAAAGAGC)
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameJurkat TCRalpha/beta alleles and CD3e and CD3z
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
543
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA