This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
DNA-CARtcr-IRESpuro
catalog :
89346
citations: 1
Reference
Taylor M, Husain K, Gartner Z, Mayor S, Vale R. A DNA-Based T Cell Receptor Reveals a Role for Receptor Clustering in Ligand Discrimination. Cell. 2017;169:108-119.e20 pubmed publisher
product information
Catalog Number :
89346
Product Name :
DNA-CARtcr-IRESpuro
article :
doi10.1016/j.cell.2017.03.006
id28189728
pubmed_id28340336
bacterial resistance :
Ampicillin
cloning :
backbonepHR
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5Mlu1
promoterSFFV
sequencing_primer_3IRES rev, WPRE rev
sequencing_primer_5SFFV for (GCTTCCCGAGCTCTATAAAAGAGC)
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameTCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagSNAPf tag
resistance markers :
543
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA