This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
DNA-CARzeta-GFP
catalog :
89344
citations: 2
Reference
Finn P, Chavez M, Chen X, Wang H, Rane D, Gurjar J, et al. Programmable Drug Control of Receptor Valency Modulates the Potency of Cell Therapeutics. bioRxiv. 2023;: pubmed publisher
Taylor M, Husain K, Gartner Z, Mayor S, Vale R. A DNA-Based T Cell Receptor Reveals a Role for Receptor Clustering in Ligand Discrimination. Cell. 2017;169:108-119.e20 pubmed publisher
product information
Catalog Number :
89344
Product Name :
DNA-CARzeta-GFP
article :
doi10.1016/j.cell.2017.03.006
id28189728
pubmed_id28340336
bacterial resistance :
Ampicillin
cloning :
backbonepHR
backbone_mutation
backbone_origin
backbone_size8311
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP
origin :
37
pi :
alt_names
Signal-Peptide-SNAPf tag- TM-CD3zeta chains fused in frame to mGFP
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5Mlu1
promoter
sequencing_primer_3GFP rev, WPRE rev (CCAGAGGTTGATTATCGATAAGC)
sequencing_primer_5SFFV for (GCTTCCCGAGCTCTATAAAAGAGC)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD3-ZETA, CD3H, CD3Q, CD3Z, CD3ZETA, IMD25, T3Z, TCRZ
geneCD247
id919
aliasesB7-2, B7.2, B70, CD28LG2, LAB72
geneCD86
id942
genbank_ids
mutation
nameDNA-CARzeta
shRNA_sequence
size1803
species
9606
Homo sapiens
tags
locationN terminal on insert
tagSNAPf tag
locationC terminal on insert
tagmEGFP
resistance markers :
543
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA