This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSG424 ELKC(S383A)
catalog :
89334
citations: 1
Reference
Whitmarsh A, Shore P, Sharrocks A, Davis R. Integration of MAP kinase signal transduction pathways at the serum response element. Science. 1995;269:403-7 pubmed
product information
Catalog Number :
89334
Product Name :
pSG424 ELKC(S383A)
article :
doi10.1126/science.7618106
id1359
pubmed_id7618106
bacterial resistance :
Ampicillin
cloning :
backbonepSG424
backbone_mutation
backbone_origin
backbone_size3396
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Elk1 cDNA encoding aa 307-428, aa383 mutated from ser-Ala, The PCR product was cut with BamH1 and Xba 1(365bp). pSG424 was cut with Bam H1 and Xba1. The Elk1 fragment was ligated into the BamH1
origin :
37
pi :
alt_names
ELK1, ETS transcription factor
cloning
clone_methodRestriction Enzyme
cloning_site_3Xba I
cloning_site_5BamHI
promoter
sequencing_primer_3pBRev Bam_primer
sequencing_primer_5SV40pro_F_primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneELK1
id2002
genbank_ids
NC_000023.11
mutationencodes aa 307-428, changed serine 383 to alanine 383
nameELK1
shRNA_sequence
size365
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGal 4
plasmid copy :
R. Marais, J. Wynne, R. Treisman, Cell 73, 381
resistance markers :
106
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA