This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET151-Vps4(101-437)-Hcp1 R325D
catalog :
89289
citations: 1
Reference
Monroe N, Han H, Shen P, Sundquist W, Hill C. Structural basis of protein translocation by the Vps4-Vta1 AAA ATPase. elife. 2017;6: pubmed publisher
product information
Catalog Number :
89289
Product Name :
pET151-Vps4(101-437)-Hcp1 R325D
article :
doi10.7554/eLife.24487
id25515
pubmed_id28379137
bacterial resistance :
Ampicillin
cloning :
backbonepET151
backbone_mutation
backbone_originInvitrogen
backbone_size5700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
origin :
37
pi :
alt_names
Vps4
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPR173C, CSC1, DID6, END13, GRD13, VPL4, VPT10
geneVPS4
id856303
genbank_ids
NM_001184270
mutationdeleted amino acids 1-100, mutated Arg-325 to Asp
nameVacuolar protein sorting-associated protein 4
shRNA_sequence
size1554
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tag6xHis-V5-TEV
locationC terminal on insert
tagHcp1
resistance markers :
2231
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA