This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPS2966
catalog :
8925
citations: 1
| Reference |
|---|
Devroe E, Erdjument Bromage H, Tempst P, Silver P. Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. J Biol Chem. 2004;279:24444-51 pubmed
|
product information
Catalog Number :
8925
Product Name :
pPS2966
article :
| doi | 10.1074/jbc.M401999200 |
| id | 330 |
| pubmed_id | 15067004 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pGEX-4T1 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | 5000 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
NDR1 ORF PCR amplified from Hela cDNA. ~1.5 kb insert digested with BamHI/ScaI and inserted into pGEX-4T1 digested with BamHI/SmaI. Completely sequenced and includes the entire NDR ORF as reported in NCBI database. Note: GST-NDR1 is extremely insoluble when expressed in E. coli, possibility that co-expression of Mob2 would help solubilize NDR1.
origin :
37
pi :
|
resistance markers :
| 480 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments