This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPS2192
catalog :
8923
citations: 1
Reference
Hood J, Hwang W, Silver P. The Saccharomyces cerevisiae cyclin Clb2p is targeted to multiple subcellular locations by cis- and trans-acting determinants. J Cell Sci. 2001;114:589-97 pubmed
product information
Catalog Number :
8923
Product Name :
pPS2192
article :
doi
id61
pubmed_id11171327
bacterial resistance :
Ampicillin
cloning :
backbonepPS1304
backbone_mutation
backbone_origin
backbone_size5200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Similar to pPS2189, but with deletion of NLS. Created by PCR amplifying the fragments of CLB2 ORF upstream (0.5kb) and downstream (0.9kb) of the bipartite NLS and joining them using KpnI ends. The KpnI site introduces a glycine and a threonine in place of the bipartite NLS. 2 micron plasmid.
origin :
37
pi :
alt_names
Clb2
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5SalI
promoter
sequencing_primer_3M13F
sequencing_primer_5M13R
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPR119W
geneCLB2
id856236
genbank_ids
mutationDeletion of CLB2 bipartite NLS (aa 183-200), KpnI site introduced at site of NLS deletion
nameClb2-deltaNLS-GFP
shRNA_sequence
size1400
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on backbone
tagGFP
resistance markers :
480
tags :
High Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA