This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPS1739
catalog :
8922
citations: 1
Reference
Ferrigno P, Posas F, Koepp D, Saito H, Silver P. Regulated nucleo/cytoplasmic exchange of HOG1 MAPK requires the importin beta homologs NMD5 and XPO1. EMBO J. 1998;17:5606-14 pubmed
product information
Catalog Number :
8922
Product Name :
pPS1739
article :
doi10.1093/emboj/17.19.5606
id327
pubmed_id9755161
bacterial resistance :
Ampicillin
cloning :
backbonepRS-GFP
backbone_mutation
backbone_origin
backbone_size5800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
pRS-GFP vector is GFP ORF and NUF2 3' UTR from pPS934 cloned into the XhoI-KpnI sites of pRS416. HOG1 (from -290 to the termination codon) was amplified by PCR using primers that replaced the termination codon with a XhoI site. CEN plasmid.
origin :
37
pi :
alt_names
HOG1-GFP
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XhoI
promoter
sequencing_primer_3M13F
sequencing_primer_5M13R
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYLR113W, SSK3
geneHOG1
id850803
genbank_ids
mutation
nameHOG1
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagGFP
resistance markers :
480
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA