pCMV-GR11 | Addgene 89105 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV-GR11

catalog :

89105

citations: 3

Reference
Pang J, Hu X, Wang Y, Liao J, Chai X, Wang X, et al. Discovery of a novel nonsteroidal selective glucocorticoid receptor modulator by virtual screening and bioassays. Acta Pharmacol Sin. 2022;: pubmed publisher
Hu X, Pang J, Zhang J, Shen C, Chai X, Wang E, et al. Discovery of Novel GR Ligands toward Druggable GR Antagonist Conformations Identified by MD Simulations and Markov State Model Analysis. Adv Sci (Weinh). 2022;9:e2102435 pubmed publisher
Pang J, Shen C, Zhou W, Wang Y, Shan L, Chai X, et al. Discovery of novel antagonists targeting the DNA binding domain of androgen receptor by integrated docking-based virtual screening and bioassays. Acta Pharmacol Sin. 2021;: pubmed publisher

product information

Catalog Number :

89105

Product Name :

pCMV-GR11

article :

doi
id	28203483
pubmed_id	8349631

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV5

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Addgene sequencing results found a 4 AA insertion after G499 and an I747T mutation compared to NCBI reference sequence NP_000167.1. The depositor has confirmed that this construct produces functional GR.

growth strain :

mammalian expression of full-length human glucocorticoid receptor in pCMV5

origin :

pi :

alt_names

hGR

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GCCR, GCR, GCRST, GR, GRL
gene	NR3C1
id	2908

genbank_ids

mutation

name

human glucocorticoid receptor

shRNA_sequence

size

2400

species

9606

Homo sapiens