tet H-ras val12 | Addgene 8900 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

tet H-ras val12

catalog :

8900

citations: 1

Reference
Chin L, Tam A, Pomerantz J, Wong M, Holash J, Bardeesy N, et al. Essential role for oncogenic Ras in tumour maintenance. Nature. 1999;400:468-72 pubmed

product information

Catalog Number :

8900

Product Name :

tet H-ras val12

article :

doi	10.1038/22788
id	319
pubmed_id	10440378

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript KS

backbone_mutation

backbone_origin

backbone_size

3350

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The construct is composed of the following elements: 1) 350bp Tet-operators with CMV minimal promoter, 2) 1.8kb rasVal12, 3) SV40 splice and polyadenylation sequences. The entire 3050bp insert containing rasV12 and regulatory elements can be released with NotI. See author's map for more information.

origin :

pi :

alt_names

ras

H-ras

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	SalI
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
gene	HRAS
id	3265

genbank_ids

mutation

G12V

name

ras val12

shRNA_sequence

size

2700

species

9606

Homo sapiens