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product summary
company name :
Addgene
product type :
cDNA
product name :
pSV Sport SREBP-1c dom neg
catalog :
8885
citations: 6
Reference
Bruschi F, Tardelli M, Herac M, Claudel T, Trauner M. Metabolic regulation of hepatic PNPLA3 expression and severity of liver fibrosis in patients with NASH. Liver Int. 2020;40:1098-1110 pubmed publisher
Armengol S, Arretxe E, Enzunza L, Llorente I, Mendibil U, Navarro Imaz H, et al. SREBP-2-driven transcriptional activation of human SND1 oncogene. Oncotarget. 2017;8:108181-108194 pubmed publisher
Dey P, Baddour J, Muller F, Wu C, Wang H, Liao W, et al. Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer. Nature. 2017;542:119-123 pubmed publisher
Yang L, Li P, Yang W, Ruan X, Kiesewetter K, Zhu J, et al. Integrative Transcriptome Analyses of Metabolic Responses in Mice Define Pivotal LncRNA Metabolic Regulators. Cell Metab. 2016;24:627-639 pubmed publisher
Yan F, Wang Q, Xu C, Cao M, Zhou X, Wang T, et al. Peroxisome proliferator-activated receptor ? activation induces hepatic steatosis, suggesting an adverse effect. PLoS ONE. 2014;9:e99245 pubmed publisher
Kim J, Spiegelman B. ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Genes Dev. 1996;10:1096-107 pubmed
product information
Catalog Number :
8885
Product Name :
pSV Sport SREBP-1c dom neg
article :
doi10.1101/gad.10.9.1096
id316
pubmed_id8654925
bacterial resistance :
Ampicillin
cloning :
backbonepSV-Sport
backbone_mutation
backbone_originInvitrogen
backbone_size3160
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
SREBP-1c cloned from a lambda gt11 rat adipocyte cDNA expression library.
origin :
37
pi :
alt_names
ADD1
SREBP
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADD-1, ADD1, SREBP-1, SREBP-1c, Srebp1
geneSrebf1
id78968
genbank_ids
mutationY305A amino acid substitution in the active version of SREBP-1c (K312R). Creates a dominant negative version of SREBP-1c.
nameSREBP-1c
shRNA_sequence
size1200
species
10116
Rattus norvegicus
tags
resistance markers :
495
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA