This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPS1622
catalog :
8854
citations: 3
Reference
Lipp N, Gautier R, Magdeleine M, Renard M, Albanèse V, Copic A, et al. An electrostatic switching mechanism to control the lipid transfer activity of Osh6p. Nat Commun. 2019;10:3926 pubmed publisher
Adam I, Jossé L, Tuite M. Human TorsinA can function in the yeast cytosol as a molecular chaperone. Biochem J. 2017;474:3439-3454 pubmed publisher
Prinz W, Grzyb L, Veenhuis M, Kahana J, Silver P, Rapoport T. Mutants affecting the structure of the cortical endoplasmic reticulum in Saccharomyces cerevisiae. J Cell Biol. 2000;150:461-74 pubmed
product information
Catalog Number :
8854
Product Name :
pPS1622
article :
doi10.1083/jcb.150.3.461
id311
pubmed_id10931860
bacterial resistance :
Ampicillin
cloning :
backbonepRS316
backbone_mutation
backbone_origin
backbone_size4895
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Constructed by amplifying SEC63 and ~400 bp of its 5' promoter from pPS599 (R. Schekman, UC Berkeley). Product cloned between XbaI and XhoI sites of CEN/URA3 plasmid, pJK50 (Kahana, MBC 1998).
origin :
37
pi :
alt_names
Sec63
pJK59
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XbaI
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYOR254C, PTL1
geneSEC63
id854428
genbank_ids
mutationsGFP=S65T V163A mutant of GFP
nameSec63-sGFP
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagGFP
resistance markers :
480
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA