This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPS1701
catalog :
8852
citations: 1
Reference
Kahana J, Schlenstedt G, Evanchuk D, Geiser J, Hoyt M, Silver P. The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B. Mol Biol Cell. 1998;9:1741-56 pubmed
product information
Catalog Number :
8852
Product Name :
pPS1701
article :
doi10.1091/mbc.9.7.1741
id310
pubmed_id9658168
bacterial resistance :
Ampicillin
cloning :
backbonena
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Integrating Nip100, 3xHA, NUF2 3'UTR plasmid. Rescues nip100delta; cin8delta. Cut with ClaI before transforming. Note that XhoI fusion site was destroyed because SalI was used to clone Nip100 (which has an internal XhoI site).
origin :
37
pi :
alt_names
pJK138
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5XbaI
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL174C, PAC13
geneNIP100
id855929
genbank_ids
mutation
nameNIP100
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
location
tag3x HA
resistance markers :
480
tags :
High Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA