This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHES795
catalog :
87943
citations: 2
Reference
Muller R, Meacham Z, Ferguson L, Ingolia N. CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes. Science. 2020;370: pubmed publisher
Aranda Díaz A, Mace K, Zuleta I, Harrigan P, El Samad H. Robust Synthetic Circuits for Two-Dimensional Control of Gene Expression in Yeast. ACS Synth Biol. 2017;6:545-554 pubmed publisher
product information
Catalog Number :
87943
Product Name :
pHES795
article :
doi10.1021/acssynbio.6b00251
id25090
pubmed_id27930885
bacterial resistance :
Ampicillin
cloning :
backbonepAAD606
backbone_mutation
backbone_origin
backbone_size7000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.
origin :
37
pi :
alt_names
ZEM
cloning
clone_methodRestriction Enzyme
cloning_site_3SacI
cloning_site_5PspOMI
promoterADH1 (crippled)
sequencing_primer_3CGCACTCACGTAAACACTTAATC
sequencing_primer_5GTGGATTCGGCTTTGGGTA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesER, ESR, ESRA, ESTRR, Era, NR3A1
geneESR1
id2099
genbank_ids
mutation
nameZif268 DBD - hER LBD - MSN2 AD
shRNA_sequence
size2173
species
9606
Homo sapiens
4932
Saccharomyces cerevisiae
100
Synthetic
tags
resistance markers :
3381
tags :
Unknown
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA