This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRK5-FLAG-ATP6V0D1
catalog :
87931
citations: 1
Reference
Zoncu R, Bar Peled L, Efeyan A, Wang S, Sancak Y, Sabatini D. mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H(+)-ATPase. Science. 2011;334:678-83 pubmed publisher
product information
Catalog Number :
87931
Product Name :
pRK5-FLAG-ATP6V0D1
article :
doi10.1126/science.1207056
id4958
pubmed_id22053050
bacterial resistance :
Ampicillin
cloning :
backbonepRK5
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Human cDNA ATP6V0D1 purchased from Open Biosystems
growth strain :
FLAG-tagged overexpression
origin :
37
pi :
alt_names
P39; VATX; VMA6; ATP6D; ATP6DV; VPATPD
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1
cloning_site_5Sal1
promoter
sequencing_primer_3pRK5-3': TGT AAC CAT TAT AAG CTG
sequencing_primer_5SP6 : ATT TAG GTG ACA CTA TAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATP6D, ATP6DV, P39, VATX, VMA6, VPATPD
geneATP6V0D1
id9114
genbank_ids
NM_004691.4
mutation
nameATP6Vod1
shRNA_sequence
size1056
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
resistance markers :
432
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA